The main factor affecting the amplitude of a nerve fiber action potential is

Astimulus intensity
Bduration of stimulus
Cconcentration of K+ on both sides of the cell membrane
Dconcentration of Na+ on both sides of the cell membrane

Correct answer: D. concentration of Na+ on both sides of the cell membrane

The amplitude of a nerve fiber action potential is primarily determined by the concentration difference of sodium ions (Na+) across the cell membrane. Changes in this concentration gradient affect the magnitude of the generated action potential.

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